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Application of Sodium Dodecyl Sulfate Micellar Electrokinetic Chromatography (SDS MEKC) for the Rapid Measurement of Aqueous Phase 2,4,6-Trinitrotoluene Metabolites in Anaerobic Sludge: A Comparison with LC/MS
- Source :
- Environmental Science & Technology. 34:2330-2336
- Publication Year :
- 2000
- Publisher :
- American Chemical Society (ACS), 2000.
-
Abstract
- The present study describes the application of sodium dodecyl sulfate micellar electrokinetic chromatography (SDS MEKC) for the fast analysis of TNT biotransformation products in the aqueous phase of anaerobic sludge cultures. SDS MEKC was performed using a Hewlett-Packard HP 3D CE capillary electrophoresis system with photodiode array detection. In a single sample injection the SDS MEKC method detected 2,4,6-trinitrotoluene (TNT), 2-hydroxylamino-4,6-dinitrotoluene (2-HADNT), 4-hydroxylamino-2,6-dinitrotoluene (4-HADNT), 2-amino-4,6-dinitrotoluene (2-ADNT), 4-amino-2,6-dinitrotoluene (4-ADNT), 2,4-diaminonitrotoluene (2,4-DANT), 2,6-diaminonitrotoluene (2,6-DANT), and 2,4,6-triaminotoluene (TAT). Analyte peaks were verified using visible/ultraviolet photodiode array spectra from commercial standards and periodic analyses using electrospray ionization liquid chromatography mass spectrometry (ES-LC-MS). A time course study constructed from the SDS MEKC data provided supporting evidence for the stepwise red...
- Subjects :
- Analyte
chemistry.chemical_compound
Chromatography
Capillary electrophoresis
chemistry
Liquid chromatography–mass spectrometry
Electrospray ionization
Metabolite
Aqueous two-phase system
Environmental Chemistry
General Chemistry
Sodium dodecyl sulfate
Micellar electrokinetic chromatography
Subjects
Details
- ISSN :
- 15205851 and 0013936X
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- Environmental Science & Technology
- Accession number :
- edsair.doi...........e63b7034d9f9a7bc80f2a073cb2879cc
- Full Text :
- https://doi.org/10.1021/es991233x