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Application of Sodium Dodecyl Sulfate Micellar Electrokinetic Chromatography (SDS MEKC) for the Rapid Measurement of Aqueous Phase 2,4,6-Trinitrotoluene Metabolites in Anaerobic Sludge: A Comparison with LC/MS

Authors :
Annamaria Halasz
Louise Paquet
Sylvie Beaudet
Jalal Hawari
Carl A. Groom
Source :
Environmental Science & Technology. 34:2330-2336
Publication Year :
2000
Publisher :
American Chemical Society (ACS), 2000.

Abstract

The present study describes the application of sodium dodecyl sulfate micellar electrokinetic chromatography (SDS MEKC) for the fast analysis of TNT biotransformation products in the aqueous phase of anaerobic sludge cultures. SDS MEKC was performed using a Hewlett-Packard HP 3D CE capillary electrophoresis system with photodiode array detection. In a single sample injection the SDS MEKC method detected 2,4,6-trinitrotoluene (TNT), 2-hydroxylamino-4,6-dinitrotoluene (2-HADNT), 4-hydroxylamino-2,6-dinitrotoluene (4-HADNT), 2-amino-4,6-dinitrotoluene (2-ADNT), 4-amino-2,6-dinitrotoluene (4-ADNT), 2,4-diaminonitrotoluene (2,4-DANT), 2,6-diaminonitrotoluene (2,6-DANT), and 2,4,6-triaminotoluene (TAT). Analyte peaks were verified using visible/ultraviolet photodiode array spectra from commercial standards and periodic analyses using electrospray ionization liquid chromatography mass spectrometry (ES-LC-MS). A time course study constructed from the SDS MEKC data provided supporting evidence for the stepwise red...

Details

ISSN :
15205851 and 0013936X
Volume :
34
Database :
OpenAIRE
Journal :
Environmental Science & Technology
Accession number :
edsair.doi...........e63b7034d9f9a7bc80f2a073cb2879cc
Full Text :
https://doi.org/10.1021/es991233x