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Improvement and Validation of<scp>d</scp>-Xylose Determination in Urine and Serum as a New Tool for the Noninvasive Evaluation of Lactase Activity in Humans
- Source :
- Journal of Clinical Laboratory Analysis. 28:478-486
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- Background The phloroglucinol assay is the current method for d-xylose determination in urine/plasma/serum. However, its sensitivity is limited when low amounts of d-xylose are to be measured, such as in the noninvasive evaluation of intestinal lactase with 4-galactosylxylose (gaxilose). An improved assay was therefore needed. Methods We developed and validated a modified version of the phloroglucinol-based assay for quantification of d-xylose in urine/serum samples. A method for gaxilose determination by gas chromatography (GC) was also optimized. Results Linearity ranged from 0.125 to 5.0 mg/l (5–200 mg/l in original sample). Accuracy at LOQ (0.125 mg/l) was 0.97/2.49% in spiked urine/serum; for other quality controls (QC), it was
- Subjects :
- Microbiology (medical)
Reproducibility
Chromatography
Intestinal lactase
Biochemistry (medical)
Clinical Biochemistry
Phloroglucinol
Public Health, Environmental and Occupational Health
Hematology
Urine
Xylose
Lactase activity
Medical Laboratory Technology
chemistry.chemical_compound
chemistry
Oral administration
Immunology and Allergy
Gas chromatography
Subjects
Details
- ISSN :
- 08878013
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Laboratory Analysis
- Accession number :
- edsair.doi...........e5fba0e6fb3f8fafd669f7e024dffd3d