Survival of Pseudomonas putida for Biodiesel Blend (B5) in Soil Bioremediation

The soil-contaminated areas can be cleaned-up by the emerging science and technology of bioremediation. This study aims to investigate the survival of Pseudomonas putida (P. putida) as biodegradable agent in soil contaminated with biodiesel/diesel blends (B5). The spillage simulation of B5 was conducted at laboratory scale for 24 days of treatment. The results show that contamination of biodiesel/diesel blends in soil induced a reduction growth of P. putida. The growth of P. putida was measured high in control compared to B5 sample proved the inhibitory effect has occurred. Nevertheless, the P. putida colonies counts for sample B5 were persisted high until the end of treatment period at 5.0 x106 CFU/ml. P. putida is very sensitive to any ecosystem perturbation therefore this study notably serves as necessary information and potentially exploits as a useful oil-soil biodegradable agent in soil bioremediation.