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Identification of Essential Genes and Synthetic Lethal Gene Combinations in Escherichia coli K-12
- Source :
- Gene Essentiality ISBN: 9781493923977
- Publication Year :
- 2015
- Publisher :
- Springer New York, 2015.
-
Abstract
- Here we describe the systematic identification of single genes and gene pairs, whose knockout causes lethality in Escherichia coli K-12. During construction of precise single-gene knockout library of E. coli K-12, we identified 328 essential gene candidates for growth in complex (LB) medium. Upon establishment of the Keio single-gene deletion library, we undertook the development of the ASKA single-gene deletion library carrying a different antibiotic resistance. In addition, we developed tools for identification of synthetic lethal gene combinations by systematic construction of double-gene knockout mutants. We introduce these methods herein.
Details
- ISBN :
- 978-1-4939-2397-7
- ISBNs :
- 9781493923977
- Database :
- OpenAIRE
- Journal :
- Gene Essentiality ISBN: 9781493923977
- Accession number :
- edsair.doi...........e4abbcfb15297b7dca8623fb3a67a680
- Full Text :
- https://doi.org/10.1007/978-1-4939-2398-4_4