Back to Search Start Over

Identification of Essential Genes and Synthetic Lethal Gene Combinations in Escherichia coli K-12

Authors :
Rikiya Takeuchi
Hitomi Dose
Hirotada Mori
Tomoya Baba
Yuta Otsuka
Kazuichi Makishi
Katsushi Yokoyama
Wataru Nomura
Barry L. Wanner
Source :
Gene Essentiality ISBN: 9781493923977
Publication Year :
2015
Publisher :
Springer New York, 2015.

Abstract

Here we describe the systematic identification of single genes and gene pairs, whose knockout causes lethality in Escherichia coli K-12. During construction of precise single-gene knockout library of E. coli K-12, we identified 328 essential gene candidates for growth in complex (LB) medium. Upon establishment of the Keio single-gene deletion library, we undertook the development of the ASKA single-gene deletion library carrying a different antibiotic resistance. In addition, we developed tools for identification of synthetic lethal gene combinations by systematic construction of double-gene knockout mutants. We introduce these methods herein.

Details

ISBN :
978-1-4939-2397-7
ISBNs :
9781493923977
Database :
OpenAIRE
Journal :
Gene Essentiality ISBN: 9781493923977
Accession number :
edsair.doi...........e4abbcfb15297b7dca8623fb3a67a680
Full Text :
https://doi.org/10.1007/978-1-4939-2398-4_4