ChemInform Abstract: A New Phosphoramidite Reagent for the Incorporation of Diazaphenoxazinone Nucleoside with Enhanced Base-Pairing Properties into Oligodeoxynucleotides

New phosphoramidite reagent 7 suitable for incorporation of dC analogue was synthesized. ODNs containing diazaphenoxaziione residues in defined positions were prepared. The stability of duplexes formed was increased up to 3-5°C per modified base. Preliminary results of molecular biological testing were reported. This work was partly supported by RFBR grant 96-04-49209, by grant HHMI#75195-544201, LLNL P99U, and grant DOE agreement No. B307902 mod. 04. The authors would like to thank Dr. Tatyana L. Azhikina for valuable help in DNA sequencing and Dr. Natalya P. Potapova for NMR spectra recording.