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Using Dynamic Changes of Chlorophyll Fluorescence in Arabidopsis thaliana to Evaluate Plant Immunity-Intensifying Bacillus spp. Strains
- Source :
- Phytopathology®. 109:1566-1576
- Publication Year :
- 2019
- Publisher :
- Scientific Societies, 2019.
-
Abstract
- The integral defense responses of plants triggered by the small molecules of plant pathogens are regarded as plant immunity. The pathogen-associated molecular pattern-triggered immunity (PTI) occurs on the recognition of a pathogen by receptors on plant cell surfaces as an infection begins. During the activation of PTI, the effectiveness of a plant’s photosynthetic system may be altered. In this study, chlorophyll fluorescence was used to assay the dynamic changes of PTI. When we used flg22Pst as an elicitor, we found that the photosynthetic electron transport rate (ETR) of Arabidopsis thaliana Col-0 was significantly decreased at 2, 4, and 24 h on treatment with a PTI-intensifying protein, plant ferredoxin-like protein (PFLP). In addition, this reduction in the photosynthetic ETR was also carried out with a PTI-intensifying Bacillus amyloliquefaciens strain, PMB05, on the induction of flg22Pst. The disease resistance against bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) was still enhanced by PMB05. Interestingly, among the eight tested Bacillus species strains, the PTI triggered by HrpNPcc from P. carotovorum subsp. carotovorum exhibited an ETR that was significantly decreased by PMB05. Furthermore, this decrease was consistent with rapid H2O2 generation and callose deposition triggered by HrpNPcc and the disease resistance against bacterial soft rot. Taken together, such results led us to conclude that the assay based on the ETR established in this study can be used as a model for evaluating the effectiveness of plant immunity-intensifying microbes for controlling plant diseases.
- Subjects :
- 0106 biological sciences
0301 basic medicine
biology
Bacillus amyloliquefaciens
fungi
Callose
food and beverages
Pectobacterium carotovorum
Plant Science
Plant disease resistance
biology.organism_classification
01 natural sciences
Elicitor
Microbiology
03 medical and health sciences
Bacterial soft rot
chemistry.chemical_compound
030104 developmental biology
chemistry
Arabidopsis thaliana
Agronomy and Crop Science
Chlorophyll fluorescence
010606 plant biology & botany
Subjects
Details
- ISSN :
- 19437684 and 0031949X
- Volume :
- 109
- Database :
- OpenAIRE
- Journal :
- Phytopathology®
- Accession number :
- edsair.doi...........e2cd092b365b53e7f9ce399d1d5f316a