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DNA sequence diversity of intergenic spacer 1 region in the non-lipid-dependent speciesMalasseziapachydermatisisolated from animals

Authors :
K. Nakagaki
Akemi Nishikawa
Takashi Sugita
S. Komori
K. Hama
Masako Takashima
A. Vollekova
J. Kucsera
Eric V. Virtudazo
V. Farkas
Kanji Takeo
J. Dorogi
E. Slavikova
Source :
Medical Mycology. 43:21-26
Publication Year :
2005
Publisher :
Oxford University Press (OUP), 2005.

Abstract

The non-lipid-dependent species Malassezia pachydermatis is frequently isolated from animals. We analyzed the DNA sequences of the intergenic spacer (IGS) 1 region, which is the most variable region in the rRNA gene, of 43 M. pachydermatis strains obtained from dogs or cats. The lengths of the IGS 1 regions ranged from 552 to 898 bp and, based on the nucleotide sequence, these IGS 1 regions were divided into three major groups with 10 subtypes. Group 1 (552-601 bp long) was characterized by the short sequence repeat (CAGCA)n and had four to 14 repeats, and Group 3 (749-898 bp long), which included the neotype strain of M. pachydermatis, was characterized by the sequence (CAGCATAACATAACACACAACA)n in the IGS1 region. Group 2 possessed partial sequences of both Groups 1 and 3. Each group shared only 41.7-55.4% similarity in the IGS1 region with the other groups. The internal transcribed spacer (ITS) region and D1/D2 26S rDNA in the rRNA gene were also sequenced for representative strains in each IGS group. The groups were distinguished by both ITS (698-712 bp long including 5.8S rDNA) and D1/D2 26S rDNA (624 bp long) sequences with sequence similarities of 91.7-96.0% and 99.7-99.0%, respectively. Our results indicate that the sequence of the IGS region of M. pachydermatis has a remarkable intraspecies diversity, compared with ITS or D1/D2 26S rDNA, and that multiple genotypic strains of M. pachydermatis colonize animal skin.

Details

ISSN :
14602709 and 13693786
Volume :
43
Database :
OpenAIRE
Journal :
Medical Mycology
Accession number :
edsair.doi...........e200120a086213f5324a4caa77277b30