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PGE2 pulsing of murine bone marrow cells reduces migration of daughter monocytes/macrophages in vitro and in vivo

Authors :
Philip Newsholme
Leonard I. Zon
Terence McGonigle
Amy R. Dwyer
Prue H. Hart
Naomi M. Scott
Helen S. Goodridge
Kevin N. Keane
Fiona J. Pixley
Eloise L Greenland
Source :
Experimental Hematology. 56:64-68
Publication Year :
2017
Publisher :
Elsevier BV, 2017.

Abstract

Monocytes/macrophages differentiating from bone marrow (BM) cells pulsed for 2 hours at 37°C with a stabilized derivative of prostaglandin E2, 16,16-dimethyl PGE2 (dmPGE2), migrated less efficiently toward a chemoattractant than monocytes/macrophages differentiated from BM cells pulsed with vehicle. To confirm that the effect on BM cells was long lasting and to replicate human BM transplantation, chimeric mice were established with donor BM cells pulsed for 2 hours with dmPGE2 before injection into marrow-ablated congenic recipient mice. After 12 weeks, when high levels (90%) of engraftment were obtained, regenerated BM-derived monocytes/macrophages differentiating in vitro or in vivo migrated inefficiently toward the chemokines colony-stimulating factor-1 (CSF-1) and chemokine (C-C motif) ligand 2 (CCL2) or thioglycollate, respectively. Our results reveal long-lasting changes to progenitor cells of monocytes/macrophages by a 2-hour dmPGE2 pulse that, in turn, limits the migration of their daughter cells to chemoattractants and inflammatory mediators.

Details

ISSN :
0301472X
Volume :
56
Database :
OpenAIRE
Journal :
Experimental Hematology
Accession number :
edsair.doi...........e01faa4acd81d8b941a9988d484513b1
Full Text :
https://doi.org/10.1016/j.exphem.2017.08.002