Investigation of the Interaction Between N-benzyl Piperidones and Bovine Serum Albumin by Spectroscopic Approaches

Fluorescence and ultraviolet spectroscopies were applied to investigate the interaction between five N-benzyl piperidones (NBP) and bovine serum albumin (BSA) under imitated physiological conditions. The experimental results show that these NBPs have a static fluorescence quenching effect on the endogenous fluorescence of BSA at the temperatures 302 and 310 K. According to the fluorescence quenching theory, the Stern–Volmer quenching constant (K SV), the binding constant (K a), and the number of binding sites (n) were obtained. Also, the effect of substituents on the binding capacity between NBP and BSA is in the order: C6H5