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Optical detection of photo-activated protein adsorption using Brewster angle microscopy
- Source :
- 2008 IEEE PhotonicsGlobal@Singapore.
- Publication Year :
- 2008
- Publisher :
- IEEE, 2008.
-
Abstract
- Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266 nm light yields the pi-pi* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with the modified quartz. The triplet state may form a ketyl radical by reaction with the solution or it may abstract a proton directly from the BSA becoming covalently tethered to the surface. The entire deposition process was monitored in real time, in-situ using a Brewster angle microscope (BAM).
Details
- Database :
- OpenAIRE
- Journal :
- 2008 IEEE PhotonicsGlobal@Singapore
- Accession number :
- edsair.doi...........de0602216b16978be0d32b6c987a2286