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Chondrogeneic Potential of MSC from Different Sources in Spheroid Culture
- Source :
- Bulletin of Experimental Biology and Medicine. 170:528-536
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- We performed a comparative study of the proliferative potential of human mesenchymal stromal cells (MSC) from three sources (tooth pulp, adipose tissue, and Wharton’s jelly) in spheroid culture; human chondroblasts served as the positive control. Histological examination revealed signs of chondrogenic differentiation in all studied cell cultures and the differences in the volume and composition of the extracellular matrix. Spheroids formed by MSC from the tooth pulp and Wharton’s jelly were characterized by low content of extracellular matrix and glycosaminoglycans. Spheroids from adipose tissue MSC contained maximum amount of the extracellular matrix and high content of glycosaminoglycans. Chondrocytes produced glycosaminoglycan-enriched matrix. Type II collagen was produced by chondrocytes (to a greater extent) and adipose tissue MSC (to a lesser extent). The results of our study demonstrate that MSC from the adipose tissue under conditions of spheroid culturing exhibited maximum chondrogenic potential.
- Subjects :
- 0301 basic medicine
Chemistry
Mesenchymal stem cell
Spheroid
Adipose tissue
General Medicine
Matrix (biology)
Chondrogenesis
General Biochemistry, Genetics and Molecular Biology
Cell biology
Extracellular matrix
Glycosaminoglycan
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Cell culture
embryonic structures
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15738221 and 00074888
- Volume :
- 170
- Database :
- OpenAIRE
- Journal :
- Bulletin of Experimental Biology and Medicine
- Accession number :
- edsair.doi...........dde1d30b3526915bba7213f567fc506e