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Certain calcium channel inhibitors exhibit a number of secondary effects on the physiological properties in
- Source :
- Functional Plant Biology. 50:195-205
- Publication Year :
- 2022
- Publisher :
- CSIRO Publishing, 2022.
-
Abstract
- An unsolved problem of contemporary plant electrophysiology is the identity of Ca2+ channels responsible for the initiation of the action potential. We took a pharmacological approach and applied several Ca2+ channel blockers (verapamil, tetrandrine, and NED-19) on a Characean (Nitellopsis obtusa) algae model system. The impact of the selected pharmaceuticals on the parameters of excitation transients of a single cell was analysed employing the two-electrode voltage clamp technique. It was revealed that tetrandrine exerted no effect, while both verapamil and NED-19 prolonged activation and inactivation durations of the excitatory Cl− current. NED-19 also significantly depolarised the excitation threshold membrane potential and shifted Ca2+ current reversal potential. Thus, NED-19 most specifically targeted Ca2+ channels. A viability assay paired with observations of cytoplasmic streaming revealed that verapamil affected not only Ca2+ channels but also exhibited non-specific effects, which eventually lead to cell death. Since many potential Ca2+ channel blockers exert additional undesirable non-specific effects, our study underlines the necessity to search for new more specific modulators of plant Ca2+ transport systems.
- Subjects :
- Plant Science
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 14454416 and 14454408
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Functional Plant Biology
- Accession number :
- edsair.doi...........ddcf7b3c8bdf987530ebc181419abc84
- Full Text :
- https://doi.org/10.1071/fp22106