Calosphaeriacanker of almond caused byCalosphaeria pulchellain Iran

Trunk diseases are a potential threat for the almond industry in Northern Iran. In a survey on fungal agents associated with almond die back and decline in East Azerbaijan province, located in Northwest of Iran, acremonium-like hyphomycete isolates with hyaline and simple conidiophores and hyaline cylindrical phialides with a finely pigmented apical region and deep, flaring collarettes were repeatedly recovered from symptomatic tissues. Fungal isolates were identified as Calosphaeria pulchella based on cultural and morphological characteristics. The identity of the species was further confirmed based on the sequence data from LSU and ITS rDNA regions. A phylogeny inferred using ITS sequence data, clustered the Calosphaeria isolates together with Calosphaeria pulchella isolates in GenBank known from Prunus persica and Prunus avium. The results of pathogenicity assay using an excised shoot method showed that Calosphaeria pulchella is highly pathogenic on almond. The distribution and host range of this new p...