Free hemoglobin reference intervals detected with hemoglobin-cyanide method on a biochemical automatic analyzer ILAB 300 PLUS

Objectives - to evaluate the reference intervals for free hemoglobin concentration with the help of hemoglobin-cyanide method and a biochemical automatic analyzer. Material and methods. The reference group consisted of 120 healthy men and 120 healthy women from the Astrakhan region aged from 20 to 60 years. The fulfilment of standard procedures during the pre-analytical prelaboratory, pre-analytical laboratory, analytical and post-analytical stages of the study ensured the quality control for free hemoglobin evaluation. Blood samples were taken with cubital vein puncture after applying a tourniquet (for not more than 1 minute), patient in lying position, using two-component blood sampling systems - disposable polypropylene tubes with a blood coagulation activator (Sarstedt, Germany). To obtain blood serum, blood tubes were centrifuged at 2500 rpm during 10 minutes. The free hemoglobin in blood serum was detected by the hemoglobin-cyanide method using a ILAB 300 PLUS biochemical automatic analyzer (Laboratory Instrumentation, USA). The data was processed using the software package Statistica 6.0 for Windows (StatSoft Inc, США). Results. We used the classical approach and followed the strict inclusion and exclusion criteria, recommended by CLSI C 28-A3 guidelines. Gender and age differences in the content of free hemoglobin in blood serum in men and women of the Astrakhan region were not found. The reference interval of free hemoglobin in men and women of the Astrakhan region was evaluated as 0.02-0.84 g/l.