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[Untitled]
- Source :
- Genome Biology. 7:R116
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- We have established a reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes.
Details
- ISSN :
- 14656906
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Genome Biology
- Accession number :
- edsair.doi...........dc30176137e5cb19e77cb17d7ceac023