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Identifying Attenuating Mutations: Tools for a New Vaccine Design against Flaviviruses
- Source :
- Intervirology. 60:8-18
- Publication Year :
- 2017
- Publisher :
- S. Karger AG, 2017.
-
Abstract
- Emerging Flaviviruses pose an increasing threat to global human health. To date, human vaccines against yellow fever virus (YFV), Japanese encephalitis virus (JEV), dengue virus (DV), and tick-borne encephalitis virus (TBEV) exist. However, there is no human vaccine against other Flaviviruses such as Zika virus (ZIKV) and West Nile virus (WNV). In order to restrict their spread and to protect populations against the diseases they induce, vaccines against these emerging viruses must be designed. Obtaining new live attenuated Flavivirus vaccines using molecular biology methods is now possible. Molecular infectious clones of the parental viruses are relatively easy to generate. Key mutations present in live attenuated vaccines or mutations known to have a key role in the Flavivirus life cycle and/or interactions with their hosts can be identified by sequencing, and are then inserted in infectious clones by site-directed mutagenesis. More recently, the use of chimeric viruses and large-scale reencoding and introduction of microRNA target sequences have also been tested. Indeed, a combination of these methods will help in designing new generations of vaccines against emerging and reemerging Flaviviruses.
- Subjects :
- 0301 basic medicine
Attenuated vaccine
viruses
Yellow fever
virus diseases
Japanese encephalitis
Biology
Dengue virus
medicine.disease
biology.organism_classification
medicine.disease_cause
Virology
Virus
3. Good health
Zika virus
03 medical and health sciences
Flavivirus
030104 developmental biology
Infectious Diseases
Veterinary virology
medicine
Subjects
Details
- ISSN :
- 14230100 and 03005526
- Volume :
- 60
- Database :
- OpenAIRE
- Journal :
- Intervirology
- Accession number :
- edsair.doi...........dbd62de08ee5d60822ebc10a6987ca26