Molecular Cloning and Sequence Analysis of Hepatic Lipase Gene in Yak

Li, S., Jiang, M., Liu, Y., Fu, X., Drackley, J.K., Chen, Y., Ye, Y., Wang, Y. and Zhong, J. 2009. Molecular cloning and sequence analysis of hepatic lipase gene in yak. J. Appl. Anim. Res., 36: 97–101. In order to study the biological function of hepatic lipase (hl) gene in yak, the cDNA sequence encoding hepatic lipase from yak was cloned by RT-PCR method using gene specific PCR primers. The cloned cDNA fragment (1540 bp) contained a 1503 bp open reading frame, encoded 500 amino acids (AAs) with a molecular mass of 56.734 kDa. It showed a high level of sequence identity to Bos Taurus (99.40%), Homo sapiens (77.64%), Oryctolagus cuniculus (76.20%), Mus musculus (68.88%), Xenopus laevis (55.27%) and Danio rerio (47.69%). The hl gene was expressed only in liver tissue and not in heart, spleen, kidney and brain tissues. Further analysis of yak HL amino acid sequence implied that it contained a complete lipase active site (VHLIGYSLGA) ranging from 162 to 171 amino acid residues. It also contained tw...