Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform

A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti-APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H2O2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC80.