The heterogeneity of the elongation factor EF1 from wheat embryos

The elongation factor EF1 from wheat embryo, can be separated in several forms with different molecular sizes. A heavy form was purified from other heterogeneous species with smaller molecular sizes. While the purified heavy form can join directly in a binary complex with GTP, it has to be converted into a light species for the formation of the ternary complex aminoacyl-tRNA-GTP-EF1.