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Laser-Induced Single-Molecule Extraction and Detection in Aqueous Poly(N-isopropylacrylamide)/1-Butanol Solutions
- Source :
- Analytical Chemistry. 93:3202-3208
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- We report photothermal phase separation of aqueous poly(N-isopropylacrylamide) (PNIPAM)/1-butanol (BuOH) solutions by focused 1064 nm laser irradiation and subsequent single microparticle formation in the solution. The single microparticle [diameter = ∼10 μm and volume = ∼picoliter (pL)] produced by laser irradiation was optically trapped by the incident 1064 nm laser beam, and this enabled us in situ Raman/fluorescence microspectroscopies of the particle. Raman spectroscopy demonstrated that the particle produced by laser irradiation was composed of PNIPAM and BuOH. In the presence of rhodamine B (RhB) in the solution, RhB was distributed from the water phase to the PNIPAM/BuOH microparticle produced by laser irradiation, as confirmed by fluorescence microspectroscopy. Laser-induced distribution/extraction of RhB to a single PNIPAM/BuOH microparticle was shown to be possible at the RhB concentration as low as 10-14 mol/dm3, where the RhB fluorescence intensity from the particle showed a step-by-step increase by every ∼3 min laser irradiation. This is the first demonstration of laser-induced simultaneous extraction and detection of single RhB molecules in solution.
- Subjects :
- Aqueous solution
010401 analytical chemistry
Analytical chemistry
Photothermal therapy
010402 general chemistry
Laser
01 natural sciences
0104 chemical sciences
Analytical Chemistry
law.invention
symbols.namesake
chemistry.chemical_compound
chemistry
law
symbols
Rhodamine B
Poly(N-isopropylacrylamide)
Irradiation
Microparticle
Raman spectroscopy
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 93
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi...........d9b5bff404b7c8e621149493c954216e