In vivo and in vitro studies on the antioxidant activity of hesperetin and its glycosylated derivatives (830.8)

The effect of of hesperetin (HT), hesperidin (HG) and G-hesperidin (GHD) on oxidative-antioxidative system and lipid profile in rats was evaluated and correlated to their in vitro antioxidant activity. Twenty-four macho wistar (n=6) fed with standard rat chow diet were supplemented with each flavonoid (1mmol/Kg) and saline solution (control group). TBARS, GPx, SOD and CAT levels were measured in hepatic tissue. The levels of triglyceride, total cholesterol and lipoprotein fraction cholesterol levels were measured in plasma. In vitro antioxidant was evaluated by DPPH radical-scavenging and the kinetic parameters of xanthine oxidase inhibition were determined by Lineweaver-Burk plots. GPx and SOD levels were significantly higher in HT and HD groups than those in control group. Both groups treated showed atherogenic index levels significantly lower compared to the control group. Hesperetin showed a greater in vitro antioxidant activity and was stronger xanthine oxidase inhibitor with IC50 value of 53µM and K...