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Functional Th1 Cells Are Required for Surgical Adhesion Formation in a Murine Model
- Source :
- The Journal of Immunology. 180:6970-6976
- Publication Year :
- 2008
- Publisher :
- The American Association of Immunologists, 2008.
-
Abstract
- Tissue trauma in the peritoneal and pelvic cavities following surgery or bacterial infection results in adhesions that are a debilitating cause of intestinal obstruction, chronic pelvic pain, and infertility in women. We recently demonstrated that CD4+ αβ T cells are essential for development of this process. Using a murine model of experimental adhesion formation, we now demonstrate that adhesion formation is characterized by the selective recruitment of Tim-3+, CCR5+, CXCR3+, IFN-γ+ cells, indicating the presence of a Th1 phenotype. We further demonstrate that adhesion formation is critically dependent on the function of Th1 cells because mice genetically deficient for IFN-γ, T-bet, or treated with Abs to the Th1-selective chemoattractant IL-16 show significantly less adhesion formation than wild-type mice. In addition, disrupting the interaction of the Th1-specific regulatory molecule Tim-3, with its ligand, significantly exacerbates adhesion formation. This enhanced response is associated with increases in the level of neutrophil-attracting chemokines KC and MIP-2, known to play a role in adhesiogenesis. These data demonstrate that the CD4+ T cells orchestrating adhesion formation are of the Th1 phenotype and delineate the central role of T-bet, Tim-3, IFN-γ, and IL-16 in mediating this pathogenic tissue response.
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 180
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi...........d8307c42837217a068e1043ec741e769