Quantitative analysis on thin-layer chromatograms an instrument and method for in situ analysis using ultraviolet light

The construction and use of an instrument designed to facilitate research into analysis by quantitation of chromatographed zones is described. The instrument determines and records on computer tape the UV transmittance at 0.5 × 0.5 mm intervals in the two dimensions of the thin layer. The application of the general K ubelka -M unk transmission equation to the interpretation of the data is described. The flexibility of the method lies with the analyst who may write computer programmes suited to his analytical problems.