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CRISPR-Cas12a trans-cleaves DNA G-quadruplexes
- Source :
- Chemical Communications. 56:12526-12529
- Publication Year :
- 2020
- Publisher :
- Royal Society of Chemistry (RSC), 2020.
-
Abstract
- We for the first time report that the activated CRISPR-Cas12a system trans-cleaves DNA G-quadruplexes (G4). The cleavage activity on human telomere G4 and TBA G4 was investigated and verified by FRET, CD, gel electrophoresis and NMR. We believe that this finding will pave a new avenue for advancing the applications of CRISPR-Cas12a and G4 in biosensing and biochemistry.
- Subjects :
- Gel electrophoresis
0303 health sciences
Aptamer
Metals and Alloys
RNA
General Chemistry
010402 general chemistry
G-quadruplex
01 natural sciences
Catalysis
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Telomere
03 medical and health sciences
chemistry.chemical_compound
Förster resonance energy transfer
Biochemistry
chemistry
Materials Chemistry
Ceramics and Composites
CRISPR
DNA
030304 developmental biology
Subjects
Details
- ISSN :
- 1364548X and 13597345
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- Chemical Communications
- Accession number :
- edsair.doi...........d6a18e67f9a5f6c9012be70879329b18