Problems in Detection ofXanthomonas oryzaepv.oryzaein Rice Seed and Potential for Improvement Using Monoclonal Antibodies

Four semiselective media that varied with respect to carbon source, amino acids, and antibiotics were evaluated for the growth of various strains of the rice bacterial blight pathogen, Xanthomonas oryzae pv. oryzae in pure and mixed culture. Plating efficiencies in pure culture ranged from 40 to 90% on these media, and colonies developed in 3-7 days, depending on the strain of X. o. oryzae. In mixtures with Erwinia herbicola and Pseudomonas putida(1:1:1), X. o. oryzae was recovered only on XOS medium. Recovery of a faster growing X. o. oryzae strain (X1-5) was improved by adding 50-100 mg/L of FeEDTA to XOS medium, but recovery of other strains was reduced or unaffected