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Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians
- Source :
- Methods in Molecular Biology ISBN: 9781493989874
- Publication Year :
- 2019
- Publisher :
- Springer New York, 2019.
-
Abstract
- Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate important physiological processes by substrate cleavage. Despite the fact that the role of calpains in cell migration and other processes has been extensively studied in vitro, the same does not apply to cell migration and morphogenetic events during embryogenesis, in vivo. Herein, we describe the use of three different methods to selectively block calpain activity in vivo in order to investigate the impact on Xenopus gastrulation and neurulation, namely, a calpain inhibitor, a dominant negative, and a morpholino antisense oligonucleotide (MO). We also provide methods to determine the effectiveness of the calpain inhibition and effect on cell fate specification and morphogenetic movements, during embryogenesis in vivo.
- Subjects :
- 0303 health sciences
biology
Morpholino
Chemistry
Convergent extension
Xenopus
Cell migration
Calpain
Cell fate determination
biology.organism_classification
Cell biology
03 medical and health sciences
0302 clinical medicine
In vivo
030220 oncology & carcinogenesis
biology.protein
Intracellular
030304 developmental biology
Subjects
Details
- ISBN :
- 978-1-4939-8987-4
- ISBNs :
- 9781493989874
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781493989874
- Accession number :
- edsair.doi...........d61019458e6adf26dc7278dbe7cb6b32