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Engineering of a cellulose synthase gene improves brown rice processing efficiency and disease resistance

Authors :
Xuewei Chen
Li Zou
Junjie Yin
Xiaobo Zhu
Mawsheng Chern
Jiang Wan
Yan Wei
Xiao Zhu
Xiang Lu
Qing Xiong
Yongyan Tang
Tuo Qi
Li Song
Yi Yang
Qingqing Hou
Cheng Zhang
Miao Yuan
Qian Wang
Hong Yuan
Jing Shao
Long Wang
Dai Ye
Mingwu Li
Hai Qing
Yu Bi
Jiali Liu
MIN HE
Wenming Wang
Shigui Li
Jing Wang
Weitao Li
Publication Year :
2022
Publisher :
Research Square Platform LLC, 2022.

Abstract

Post-harvest processing efficiency and in-field disease resistance are two major traits for crop improvement. Higher grain-processing efficiency saves energy while stronger disease resistance reduces pesticide usage contributing to human health and environmental safety. However, simultaneous improvement on these two traits is exceptionally difficult due to the lack of suitable germplasm/gene resources and technologies. To address this problem in rice, we screened a rice mutant population and identified a rice mutant, improved brown rice processing efficiency 1 (ipe1), which has OsCesA7 mutated leading to improved brown rice processing efficiency. However, ipe1 shows reduced disease resistance. To restore disease resistance in ipe1 but keep its improved grain processing efficiency, we identified a rice intrinsic histone deacetylase 705 (HDA705) gene promoter, which has a similar expression pattern as the OsCesA7 promoter except with little expression in rice glumes, and expressed OsCesA7 with this promoter (proHDA705:OsCesA7). The proHDA705:OsCesA7 lines carry enhanced disease resistance and elevated brown rice processing efficiency with no compromises in other agronomic traits. This engineering strategy greatly saves energy used for brown rice processing and reduces pesticide usage, contributing not only to grain yield but also to human health, environmental safety, and energy saving.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........d55010213d8735e8a7fa4cad04968e78
Full Text :
https://doi.org/10.21203/rs.3.rs-1823162/v1