In-vivo Histopathological Efficacy study of Hinguleshwar rasa and Indomethacin on FCA induced Rheumatoid Arthritis in Paw model of Rats

As per Ayurveda symptoms of Rheumatoid arthritis resembles with Amavata disease. Action of Hinguleshwara rasa on Amavata is mentioned in the text of Rasatarangini in Ayurveda. In this study investigation was made to find out preventive & curative changes occurred in rat paw of Freuds Complete Adjuvant induced RA model. Indomethacin was kept as standard control and Hinguleshwar rasa as a treatment. After induction of arthritis with FCA on day 14, animals were treated up to 28th day of the study with indomethacin and Hinguleshwar rasa. After completion of 28 days of daily single oral dose administration of Hinguleshwar rasa and Indomethacin, animals were sacrificed. Ankle joints were severed and processed for histological studies. It was observed that the joints of Hinguleshwar rasa treated animals showed recovery towards normal histological architecture whereas all other joint samples of bones of rats were showing focal and minimal pathological changes, focal and minimal congestion of blood vascular tissue. Similar normal Histo-architecture was found in Indomethacin treated animals. However it was observed that, Hinguleshwar rasa showed better restoration of joint Histopathological changes occurred due to FCA damage. Hence the efficacy of Hinguleshwar rasa on Amavat (Rheumatoid arthritis) was better than Indomethacin treatment.