Effect of K+ Depletion on Glutamate Dehydrogenase

Slices of renal cortices were homogenized in Trisolv (Bioteck Laboratories, Houston,Tex., USA) at an approximate ratio of 1 g tissue/10 ml. RNA was precipitated with isopro-panol and washed twice with 75% ethanol. The electrophoresis of RNA was performed in1% agarose-formaldehyde gel, with 20 lg of RNA sample in each lane. After the electrophor-esis, RNA was transferred to a nylon membrane (maximum strength Nytran; Schleicher &Schuell, Keene, N.H., USA) with vacuum blotting. The membrane was prehybridized for2 h with 4¶standard saline citrate (SSC), 5¶Denhart’s solution, 40% formamide, 2.5% SDS,1¶blocking reagent (Bioteck Laboratories), 5% dextran, 0.05 mg/ml sheared salmon sperm,0.1 mg/ml Poly C, 0.05 mg/ml Torula yeast RNA and 20 m