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A novel hepatoma-specific fluorescent probe for imaging endogenous peroxynitrite in live HepG2 cells
- Source :
- Sensors and Actuators B: Chemical. 289:124-130
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Cell-selective imaging of some biological species is significant for the diseases research of a specific tissue or organ. Peroxynitrite (ONOO−) as a kind of reactive nitrogen species (RNS), plays extremely important roles in diverse physiological and pathological processes. Developing cell-specific fluorescent probes for tracing ONOO− in living systems remains a great challenge due to the complexity of internal environment and high autofluorescence. In this work, we developed a galactose-appended hepatoma-specific fluorescent probe Gal-NHP with hydroxynaphthalimide as the fluorophore and hydroquinone moiety as the responsive receptor for sensitively detecting endogenous ONOO− in live HepG2 cells. Probe Gal-NHP could selectively trace ONOO− without interference from other species such as HOCl and H2O2. In addition, the introduction of galactose enhanced the water solubility of this probe, and Gal-NHP could respond quickly to ONOO− in aqueous solution. The quantitative experiment showed the excellent sensitivity of this probe with a good linear relationship. Above all, fluorescence imaging results displayed it possessed excellent hepatoma-specific ability and could successfully detect endogenous ONOO− of hepatoma cells in real time. Thus, this newly developed probe would benefit to reveal the roles of ONOO− in hepatoma cells.
- Subjects :
- inorganic chemicals
Fluorescence-lifetime imaging microscopy
Fluorophore
Endogeny
02 engineering and technology
010402 general chemistry
01 natural sciences
chemistry.chemical_compound
Materials Chemistry
Electrical and Electronic Engineering
Receptor
Instrumentation
Reactive nitrogen species
Chemistry
Metals and Alloys
021001 nanoscience & nanotechnology
Condensed Matter Physics
Fluorescence
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Autofluorescence
cardiovascular system
Biophysics
0210 nano-technology
Peroxynitrite
Subjects
Details
- ISSN :
- 09254005
- Volume :
- 289
- Database :
- OpenAIRE
- Journal :
- Sensors and Actuators B: Chemical
- Accession number :
- edsair.doi...........d268e8d455c558c4c592862baa15fe28