Additional volatile constituents ofArtemisia vulgaris l. herb

The extract obtained by solid-liquid extraction with pentane-dichloromethane (2+1, v/v) from dry mugwort (Artemisia vulgaris L.) herb was fractionated by means of gel permeation chromatography (GPC) and subsequent middle-pressure liquid chromatography (MPLC). Capillary gas chromatography (HRGC) and combined HRGC techniques, i.e. HRGC-mass spectrometry (HRGC-MS) and HRGC-Fourier transform infrared spectroscopy (HRGC-FTIR) of the separated MPLC fractions revealed the occurrence of 54 additional volatiles not described as yet in A. vulgaris herb. Among them, chiral evaluation of y-nonalactone carried out by on-line coupled multidimensional gas chromatography-mass spectrometry (MDGC-MS) revealed a distribution of (R): (S) = 34:66%.