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Micro-computed tomography as a platform for exploring Drosophila development
- Source :
- Development.
- Publication Year :
- 2019
- Publisher :
- The Company of Biologists, 2019.
-
Abstract
- Understanding how events at the molecular and cellular scales contribute to tissue form and function is key to uncovering the mechanisms driving animal development, physiology and disease. Elucidating these mechanisms has been enhanced through the study of model organisms and the use of sophisticated genetic, biochemical and imaging tools. Here, we present an accessible method for non-invasive imaging of Drosophila melanogaster at high resolution using micro-computed tomography (µ-CT). We show how rapid processing of intact animals, at any developmental stage, provides precise quantitative assessment of tissue size and morphology, and permits analysis of inter-organ relationships. We then use µ-CT imaging to study growth defects in the Drosophila brain through the characterization of abnormal spindle (asp) and WD repeat domain 62 (Wdr62), orthologs of the two most commonly mutated genes in human microcephaly patients. Our work demonstrates the power of combining µ-CT with traditional genetic, cellular and developmental biology tools available in model organisms to address novel biological mechanisms that control animal development and disease.
- Subjects :
- 0303 health sciences
Developmental stage
biology
ved/biology
Micro computed tomography
ved/biology.organism_classification_rank.species
Computational biology
biology.organism_classification
03 medical and health sciences
0302 clinical medicine
Form and function
Drosophila melanogaster
Model organism
Molecular Biology
Gene
Developmental biology
Drosophila
030217 neurology & neurosurgery
030304 developmental biology
Developmental Biology
Subjects
Details
- ISSN :
- 14779129 and 09501991
- Database :
- OpenAIRE
- Journal :
- Development
- Accession number :
- edsair.doi...........d1bf72a9b7b338ff3d0393e02c04b3fe