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[40] Chemical modification of rhodopsin with imidoesters: Synthesis of reagents, membrane permeability of reagents, and modification methods

[40] Chemical modification of rhodopsin with imidoesters: Synthesis of reagents, membrane permeability of reagents, and modification methods

Authors :
Edward A. Dratz
George P. Miljanich
Peter Nemes
Publication Year :
1982
Publisher :
Elsevier, 1982.

Abstract

Publisher Summary This chapter discusses the chemical modification of rhodopsin with imidoesters: synthesis of reagents, membrane permeability of reagents, and modification methods. Chemical modification of biological macromolecules has yielded a wealth of information with regard to their structure and function. For example, covalent labeling of membranes by membrane-permeable and impermeable reagents provides a useful probe for studying the molecular architecture of these structures. This chapter describes the use of this approach in the study of the topological arrangement of rhodopsin within the rod outer segment (ROS) disk membrane. The reagents employed were isethionyl acetimidate (IAI), an imidoester first developed to investigate the structure of the erythrocyte membrane, and two newly synthesized imidates, ethyl acetimidate (SAI) and choline acetimidate (CAI). IAI and CAI are charged molecules and hence have relatively low membrane permeabilities, whereas SAI is highly membrane permeable.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........d16bd13a0f20f66d6f4dee9ba6188c99
Full Text :
https://doi.org/10.1016/s0076-6879(82)81042-2