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Reverse line blot hybridisation screening of Pseudallescheria/Scedosporium species in patients with cystic fibrosis
- Source :
- Mycoses. 54:5-11
- Publication Year :
- 2011
- Publisher :
- Wiley, 2011.
-
Abstract
- The PCR-RLB (reverse line blot hybridisation) was applied as a molecular technique for the detection of members of Pseudallescheria and Scedosporium from sputum of patients with cystic fibrosis (CF). Fifty-nine sputum samples were collected from 52 CF patients, which were analysed by culture and PCR-RLB. Conventional and semi-selective culture yielded five positive samples, but the PCR-RLB hybridisation assay permitted the detection of members of Pseudallescheria/Scedosporium in 32 out of 52 patients (61.5%). In total, PCR-RLB yielded 47 positives. Pseudallescheria apiosperma was detected in 20 samples, while Pseudallescheria boydii and Pseudallescheria aurantiacum were detected in 17 and eight samples, respectively. Six samples gave a positive reaction with two distinct species-specific probes and one sample with three probes. In conclusion, the PCR-RLB assay described in this study allows the detection of Scedosporium spp. in CF sputum samples and the identification of Pseudallescheria apiosperma, P. boydii, S. aurantiacum, Scedosporium prolificans and Pseudallescheria minutispora.
- Subjects :
- 0303 health sciences
biology
Scedosporium prolificans
030306 microbiology
Pseudallescheria
Dermatology
General Medicine
biology.organism_classification
medicine.disease
Cystic fibrosis
Microbiology
Scedosporium
Pseudallescheria boydii
Blot
03 medical and health sciences
Infectious Diseases
medicine
Sputum
In patient
medicine.symptom
030304 developmental biology
Subjects
Details
- ISSN :
- 09337407
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Mycoses
- Accession number :
- edsair.doi...........d1681934939382a522d6ddec688cb937