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Pseudomethylobacillus aquaticus gen. nov., sp. nov., a new member of the family Methylophilaceae isolated from an artificial reservoir
- Source :
- International Journal of Systematic and Evolutionary Microbiology. 69:3551-3559
- Publication Year :
- 2019
- Publisher :
- Microbiology Society, 2019.
-
Abstract
- A bacterial strain, designated H-5T, was isolated from an artificial reservoir in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain H-5T were Gram-stain-negative, aerobic, motile by means of a single polar flagellum, rod-shaped, covered by large capsules and formed white colonies. Growth occurred at 15–30 °C (optimum, 25 °C), at pH 6–8 (optimum, pH 7) and with 0–0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on the 16S rRNA gene, the methanol dehydrogenase gene and the coding sequences of 92 protein clusters indicated that strain H-5T was affiliated with genera in the family Methylophilaceae in the class Betaproteobacteria . Strain H-5T was most closely related to Methylobacillus methanolivorans ZT with a 95.0 % 16S rRNA gene sequence similarity. Strain H-5T showed less than 73.7 % average nucleotide identity and less than 23.6 % digital DNA–DNA hybridization identity compared to the strains of related genera within the family Methylophilaceae . The predominant fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The major isoprenoid quinone was Q-8 and the DNA G+C content was 58.3 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, one uncharacterized aminophospholipid, one uncharacterized phospholipid and one uncharacterized lipid. On the basis of the genotypic and phenotypic data presented here, strain H-5T represents a novel species of a new genus in the family Methylophilaceae , for which the name Pseudomethylobacillus aquaticus gen. nov., sp. nov. is proposed. The type strain is H-5T (=BCRC 81154T=KCTC 62865T).
- Subjects :
- 0106 biological sciences
0301 basic medicine
biology
Phylogenetic tree
Methanol dehydrogenase
Strain (chemistry)
030106 microbiology
General Medicine
Methylophilaceae
16S ribosomal RNA
biology.organism_classification
010603 evolutionary biology
01 natural sciences
Microbiology
03 medical and health sciences
Genus
Gene
Ecology, Evolution, Behavior and Systematics
Betaproteobacteria
Subjects
Details
- ISSN :
- 14665034 and 14665026
- Volume :
- 69
- Database :
- OpenAIRE
- Journal :
- International Journal of Systematic and Evolutionary Microbiology
- Accession number :
- edsair.doi...........d111cf1ccd6382d5e2d374ab13f26676