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Stereoselective quenching of cedryl carbocation in epicedrol biosynthesis
- Source :
- Tetrahedron Letters. 57:1161-1164
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Epicedrol synthase catalyzes the cyclization of achiral diphosphate substrate, ( E , E )-farnesyldiphosphate (FPP) into epicedrol. GC–MS analysis of assay extracts obtained by incubating FPP with epicedrol synthase in 21.6 at % H 2 18 O buffer showed the molecular ion of 224 for epicedrol. The labeled oxygen study presented here unambiguously demonstrates that the hydroxyl group of the epicedrol synthase enzymatic product, epicedrol, is derived from a water molecule, not from the phosphate moiety of the FPP.
- Subjects :
- 0301 basic medicine
Quenching (fluorescence)
ATP synthase
biology
Stereochemistry
Organic Chemistry
Substrate (chemistry)
Carbocation
Biochemistry
Isotopic labeling
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Biosynthesis
chemistry
Drug Discovery
biology.protein
Moiety
Organic chemistry
Stereoselectivity
Subjects
Details
- ISSN :
- 00404039
- Volume :
- 57
- Database :
- OpenAIRE
- Journal :
- Tetrahedron Letters
- Accession number :
- edsair.doi...........d0bdd3faeebf1e4f2187494856ac029d