Identification of the cyanide stretching frequency in the cyano derivative of copper/zinc-superoxide dismutase by IR and Raman spectroscopy

Cyanide has been investigated as a potential ligand-directed probe of the coordination chemistry of Cu(II) and Cu(I) active sites via vibrational spectroscopic studies of CN - coordinated to the metal centers in non-blue copper proteins. Native superoxide dismutase (SOD) was found to bind one CN - that had IR and Raman frequencies at 2137 cm -1