Saponins and polar compounds from Trifolium resupinatum

In addition to the known soyasaponin I, 3- O -[α- l -rhamnopyranosyl(1→2)-β- d -galactopyranosyl(1→2-β- d -glucuronopyranosyl]-22- O -[β- d -glucuronopyranosyl(1→2)-β- d -glucopyranosyl]soyasapogenol B, soyasaponin II, 3- O -β- d -glucopyranosyl sitosterol, 3- O -β- d -glucopyranosyl stigmasterol and 3- O -α- l -arabinopyranosyl-(1→6)-β- d -glucopyranosyl]oct-1-ene-3-ol, the new saponin, 3- O -α- l -rhamnopyranosyl(1→2)-β- d -galactopyranosyl(1→2)-β- d -glucuronopyranosyl]-22- O -β- d -glucopyranosyl soyasapogenol B ( 2 ) was isolated and characterised from the seed saponins fraction of Trifolium resupinatum . The structural determination is based on spectroscopic methods (including DQF-COSY, HETCOR, TOCSY, ROESY and NOEs experiments).