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A microconductometric ethanol sensor prepared through encapsulation of alcohol dehydrogenase in chitosan: application to the determination of alcoholic content in headspace above beverages
- Source :
- Journal of Materials Science: Materials in Electronics. 32:17752-17763
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- A conductometric transducer is proposed for the first time for the detection of ethanol vapor. This ethanol microsensor is prepared by encapsulation of alcohol dehydrogenase (ADH) in chitosan. Interdigitated electrodes fabricated by silicon technology were used. The electrodeposition of chitosan allows the addressing of the chitosan film on the microconductometric devices and to encapsulate ADH and nicotinamide adenine dinucleotide (NAD+), which was monitored by FTIR. The analytical performance of the ethanol microsensor was determined in gaseous methanol, ethanol, and acetone samples, collected from the headspace above aqueous solutions of known concentration. The response time (tRec) of the sensor varies from 7 to 21 s from lower concentrations to higher concentrations. The detection limit is 0.12v/v % in the gas phase, corresponding to 0.22 M in the liquid phase. The relative standard deviation for the same sensor is from 12% for lower concentrations to 2% for higher concentrations. The ethanol sensor presents 2.6 times lower sensitivity for methanol and 28.3 times lower sensitivity for acetone. A detection of ethanol in the headspace of a red wine sample lead to an alcohol content in good agreement with the value given by the producer.
- Subjects :
- 010302 applied physics
Detection limit
Materials science
Aqueous solution
Ethanol
Chromatography
Conductometry
biology
technology, industry, and agriculture
Condensed Matter Physics
01 natural sciences
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Chitosan
chemistry.chemical_compound
chemistry
0103 physical sciences
Acetone
biology.protein
Methanol
Electrical and Electronic Engineering
Alcohol dehydrogenase
Subjects
Details
- ISSN :
- 1573482X and 09574522
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Journal of Materials Science: Materials in Electronics
- Accession number :
- edsair.doi...........ce66392187dd2102aab9d965ebcd06cc