Multiple lectins as major proteins in the coelomic fluid of the acorn barnacle Megabalanus rosa

An enzyme-linked immunosorbent assay for the multiple lectins (BRA-2, BRA-3) of the acorn barnacle Megabalanus rosa was developed. The assay was used to study the multiple lectin levels in the coelomic fluid and various tissue homogenates of M. rosa. The concentrations of the multiple lectins ranged from 0.13 to 2.2 mg/ml for BRA-2 and from 0.07 to 0.16 mg/ml for BRA-3, respectively. The protein concentration of the coelomic fluid ranged from 1.4 to 4.8 mg/ml. The multiple lectins in the coelomic fluid were also identified as the major proteins by two-dimensional gel electrophoresis.