Back to Search Start Over

Reconstruction algorithm using 2N+1 raw images for structured illumination microscopy

Authors :
Xiang Fang
Kai Wen
Sha An
Juanjuan Zheng
Jianlang Li
Zeev Zalevsky
Peng Gao
Source :
Journal of the Optical Society of America A. 40:765
Publication Year :
2023
Publisher :
Optica Publishing Group, 2023.

Abstract

This paper presents a structured illumination microscopy (SIM) reconstruction algorithm that allows the reconstruction of super-resolved images with 2N + 1 raw intensity images, with N being the number of structured illumination directions used. The intensity images are recorded after using a 2D grating for the projection fringe and a spatial light modulator to select two orthogonal fringe orientations and perform phase shifting. Super-resolution images can be reconstructed from the five intensity images, enhancing the imaging speed and reducing the photobleaching by 17%, compared to conventional two-direction and three-step phase-shifting SIM. We believe the proposed technique will be further developed and widely applied in many fields.

Details

ISSN :
15208532 and 10847529
Volume :
40
Database :
OpenAIRE
Journal :
Journal of the Optical Society of America A
Accession number :
edsair.doi...........cdcf7933c4ca088e0e3f1fa399c6afa2
Full Text :
https://doi.org/10.1364/josaa.483884