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Reconstruction algorithm using 2N+1 raw images for structured illumination microscopy
- Source :
- Journal of the Optical Society of America A. 40:765
- Publication Year :
- 2023
- Publisher :
- Optica Publishing Group, 2023.
-
Abstract
- This paper presents a structured illumination microscopy (SIM) reconstruction algorithm that allows the reconstruction of super-resolved images with 2N + 1 raw intensity images, with N being the number of structured illumination directions used. The intensity images are recorded after using a 2D grating for the projection fringe and a spatial light modulator to select two orthogonal fringe orientations and perform phase shifting. Super-resolution images can be reconstructed from the five intensity images, enhancing the imaging speed and reducing the photobleaching by 17%, compared to conventional two-direction and three-step phase-shifting SIM. We believe the proposed technique will be further developed and widely applied in many fields.
Details
- ISSN :
- 15208532 and 10847529
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Journal of the Optical Society of America A
- Accession number :
- edsair.doi...........cdcf7933c4ca088e0e3f1fa399c6afa2
- Full Text :
- https://doi.org/10.1364/josaa.483884