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Pathological lesions in the central nervous system and peripheral tissues of ddY mice with street rabies virus (1088 strain)

Authors :
Akira Nishizono
Kentaro Yamada
Satoshi Inoue
Kazunori Kimitsuki
Nozomi Shiwa
Chun-Ho Park
Source :
Journal of Veterinary Medical Science. 79:970-978
Publication Year :
2017
Publisher :
Japanese Society of Veterinary Science, 2017.

Abstract

Most studies on rabies virus pathogenesis in animal models have employed fixed rabies viruses, and the results of those employing street rabies viruses have been inconsistent. Therefore, to clarify the pathogenesis of street rabies virus (1088 strain) in mice, 106 focus forming units were inoculated into the right hindlimb of ddY mice (6 weeks, female). At 3 days postinoculation (DPI), mild inflammation was observed in the hindlimb muscle. At 5 DPI, ganglion cells in the right lumbosacral spinal dorsal root ganglia showed chromatolysis. Axonal degeneration and inflammatory cells increased with infection progress in the spinal dorsal horn and dorsal root ganglia. Right hindlimb paralysis was observed from 7 DPI, which progressed to quadriparalysis. However, no pathological changes were observed in the ventral horn and root fibers of the spinal cord. Viral antigen was first detected in the right hindlimb muscle at 3 DPI, followed by the right lumbosacral dorsal root ganglia, dorsal horn of spinal cord, left red nuclei, medulla oblongata and cerebral cortex (M1 area) at 5 DPI. These results suggested that the 1088 virus ascended the lumbosacral spinal cord via mainly afferent fibers at early stage of infection and moved to cerebral cortex (M1 area) using descending spinal tract. Additionally, we concluded that significant pathological changes in mice infected with 1088 strain occur in the sensory tract of the spinal cord; this selective susceptibility results in clinical features of the disease.

Details

ISSN :
13477439 and 09167250
Volume :
79
Database :
OpenAIRE
Journal :
Journal of Veterinary Medical Science
Accession number :
edsair.doi...........cdc80ed078efaa289d09cd29f4903c98
Full Text :
https://doi.org/10.1292/jvms.17-0028