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A ratiometric fluorescent sensor for pH fluctuation and its application in living cells with low dark toxicity
- Source :
- Dyes and Pigments. 136:522-528
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- It is highly demanded and challenging to develop small-molecular fluorescent sensors for the ratiometric detection of pH fluctuation. A ratiometric fluorescent pH sensor was constructed by integrating a pH-sensitive fluorophore with a pH-insensitive hemicyanine group. Its linear and reversible ratiometric fluorescent response from pH 7.0 to 8.8 and 6.0 to 9.0 respectively makes this sensor suitable for the practical tracking of pH fluctuation in live cells. With this sensor, stimulated pH fluctuation has been successfully tracked in a ratiometric manner via fluorescence imaging with low dark toxicity.
- Subjects :
- Fluorescence-lifetime imaging microscopy
Fluorophore
Chemistry
Process Chemistry and Technology
General Chemical Engineering
02 engineering and technology
010402 general chemistry
021001 nanoscience & nanotechnology
Photochemistry
01 natural sciences
Fluorescence
0104 chemical sciences
chemistry.chemical_compound
0210 nano-technology
Subjects
Details
- ISSN :
- 01437208
- Volume :
- 136
- Database :
- OpenAIRE
- Journal :
- Dyes and Pigments
- Accession number :
- edsair.doi...........cd55f789c7392f92779ee0f577f8efd2