Rapid Determination of Sulfathiazole, Oxytetracycline and Tetracycline in Honey by High-Performance Liquid Chromatography

A simple, rapid and precise analytical method for sulfathiazole, oxytetracycline and tetracycline residues in honey has been established using micellar reversed-phase high-performance liquid chromatography with photometric detection. The mean recoveries of sulfathiazole, oxytetracycline and tetracycline were 97.7, 95.3 and 100.4%, respectively. Detection limits in honey were 0.9 μg/g for sulfathiazole, 1.2 μg/g for oxytetracycline and 3.0 μg/g for tetracycline.