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Specific MicroRNAs Found in Extracellular Matrix Vesicles Regulate Proliferation and Differentiation in Growth Plate Chondrocytes
- Source :
- Calcified Tissue International. 109:455-468
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Matrix vesicles (MVs) are extracellular organelles produced by growth plate cartilage cells in a zone-specific manner. MVs are similar in size to exosomes, but they are tethered to the extracellular matrix (ECM) via integrins. Originally associated with matrix calcification, studies now show that they contain matrix processing enzymes and microRNA that are specific to their zone of maturation. MVs produced by costochondral cartilage resting zone (RC) chondrocytes are enriched in microRNA 503 whereas those produced by growth zone (GC) chondrocytes are enriched in microRNA 122. MVs are packaged by chondrocytes under hormonal and factor regulation and release of their contents into the ECM is also under hormonal control, suggesting that their microRNA might have a regulatory role in growth plate proliferation and maturation. To test this, we selected a subset of these enriched microRNAs and transfected synthetic mimics back into RC and GC cells. Transfecting growth plate chondrocytes with select microRNA produced a broad range of phenotypic responses indicating that MV-based microRNAs are involved in the regulation of these cells. Specifically, microRNA 122 drives both RC and GC cells toward a proliferative phenotype, stabilizes the matrix and inhibits differentiation whereas microRNA 22 exerts control over regulatory factor production. This study demonstrates the strong regulatory capability possessed by unique MV enriched microRNAs on growth plate chondrocytes and their potential for use as therapeutic agents.
- Subjects :
- 0301 basic medicine
biology
Chemistry
Endocrinology, Diabetes and Metabolism
Cartilage
Integrin
030209 endocrinology & metabolism
Transfection
Matrix (biology)
Microvesicles
Cell biology
Extracellular matrix
03 medical and health sciences
0302 clinical medicine
Endocrinology
medicine.anatomical_structure
microRNA
Extracellular
medicine
biology.protein
Orthopedics and Sports Medicine
030101 anatomy & morphology
Subjects
Details
- ISSN :
- 14320827 and 0171967X
- Volume :
- 109
- Database :
- OpenAIRE
- Journal :
- Calcified Tissue International
- Accession number :
- edsair.doi...........cc7b9c4926b4e2363c3cd2c1cac7d740