Analysis and Characterization of Anthocyanin from Phalsa (Grewia asiatica)

Anthocyanins ACNs are water soluble plant pigments having a vital role in plant physiology and human health Grewia asiatica L genus Tiliaceae is a fruit producing shrub originating from south Asia Pakistan India East Cambodia cultivated mainly for its comestible fruit and well reported diverse medicinal uses The current study was the first attempt to characterize ACNs from G asiatica native to Pakistan Results revealed that maximum anthocyanin extraction yield micro g g was obtained with acidified methanol as compared to other investigated solvents water methanol ethanol and their binary mixture Total seven ACNs were identified including non acylated delphinidin O glucoside peonidin O glucoside pelargonidin O malonyl glucoside acylated cyanidin peonidin and pelargonidin O rdquo acetylglucoside and pyranoanthocyanin Malvidin O glucoside pyruvic acid Among them cyanidin O quot acetylglucoside was the major ACN comprising micro g g of total ACNs composition followed by peonidin O glucoside consisting of micro g g and pelargonidin O rdquo acetyl glucoside micro g g Moreover the study of ACNs stability at the temperature deg C testified the better temperature tolerance by ACNs under dark at temperature ranging deg C Hence the results revealed that G asiatica is a potent fruit rich in ACNs and can be used as food colorant and nutraceutical