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HLA-A2.1-Associated Peptides from a Mutant Cell Line: A Second Pathway of Antigen Presentation

Authors :
Hanspeter Michel
Jeffrey Shabanowitz
Robert A. Henderson
Kazuyasu Sakaguchi
Donald F. Hunt
Ettore Appella
Victor H. Engelhard
Source :
Science. 255:1264-1266
Publication Year :
1992
Publisher :
American Association for the Advancement of Science (AAAS), 1992.

Abstract

Peptides extracted from HLA-A2.1 class I major histocompatibility complex (MHC) molecules expressed on the antigen processing mutant CEMx721.174.T2 were characterized by electrospray ionization-tandem mass spectrometry. Only seven dominant peptides were found, in contrast to over 200 associated with HLA-A2.1 on normal cells. These peptides were derived from the signal peptide domains of normal cellular proteins, were usually larger than nine residues, and were also associated with HLA-A2.1 in normal cells. These results suggest that proteolysis of signal peptide domains in the endoplasmic reticulum is a second mechanism for processing and presentation of peptides for association with class I molecules.

Details

ISSN :
10959203 and 00368075
Volume :
255
Database :
OpenAIRE
Journal :
Science
Accession number :
edsair.doi...........cba9e1cf9b0f7110bc4583f5be874ca3
Full Text :
https://doi.org/10.1126/science.1546329