Behavior of Endothelial Cells Regulated by a Dynamically Changed Microenvironment of Biodegradable PLLA-PC

The influence of PLLA-PC membranes on the behavior of vascular ECs is studied. It was found that the membranes were not cytotoxic. The cell adhesion and spread on PLLA-PC 30/1 were initially depressed. After 1 d, however, cells attached and spread on the copolymer, and proliferated beyond 2 d. To elucidate the mechanism of this behavior, the surface morphology and the degradation products of the PLLA/PC 30/1 membrane during the culture period were tested, and Fg adsorption to the membranes was examined. It is concluded that the behavior of ECs can be regulated by a dynamically changed microenvironment of the PLLA-PC membrane. PLLA-PC copolymers might therefore serve as a novel material for tissue engineering.