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Single-cell analysis of human adipose tissue identifies depot- and disease-specific cell types
- Source :
- Nature Metabolism. 2:97-109
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- The complex relationship between metabolic disease risk and body fat distribution in humans involves cellular characteristics which are specific to body fat compartments. Here we show depot-specific differences in the stromal vascual fraction of visceral and subcutaneous adipose tissue by performing single-cell RNA sequencing of tissue specimen from obese individuals. We characterize multiple immune cells, endothelial cells, fibroblasts, adipose and hematopoietic stem cell progenitors. Subpopulations of adipose-resident immune cells are metabolically active and associated with metabolic disease status and those include a population of potential dysfunctional CD8+ T cells expressing metallothioneins. We identify multiple types of adipocyte progenitors that are common across depots, including a subtype enriched in individuals with type 2 diabetes. Depot-specific analysis reveals a class of adipocyte progenitors unique to visceral adipose tissue, which shares common features with beige preadipocytes. Our human single-cell transcriptome atlas across fat depots provides a resource to dissect functional genomics of metabolic disease.
- Subjects :
- 0303 health sciences
Cell type
education.field_of_study
Stromal cell
Endocrinology, Diabetes and Metabolism
Population
Adipose tissue
Hematopoietic stem cell
Cell Biology
Biology
Cell biology
Transcriptome
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine.anatomical_structure
chemistry
Physiology (medical)
Adipocyte
Internal Medicine
medicine
Progenitor cell
education
030217 neurology & neurosurgery
030304 developmental biology
Subjects
Details
- ISSN :
- 25225812
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Nature Metabolism
- Accession number :
- edsair.doi...........cacbc823a4b47d618ffb4663bedf025e