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Use of Myxococcus xanthus protoporphyrinogen oxidase as a selectable marker for transformation of rice
- Source :
- Pesticide Biochemistry and Physiology. 88:31-35
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Protoporphyrinogen oxidase (PPO) is the target enzyme of peroxidizing herbicides. The overexpression of Myxococcus xanthus PPO ( Mx PPO) confers a high level of herbicide resistance in rice. Among the peroxidizing herbicides, butafenacil has an efficiency ∼1000-fold that of oxadiazon, as judged by calli susceptibility tests upon herbicide treatment. Butafenacil (0.1 μM) was used to select transgenic rice plants expressing Mx PPO under the control of the constitutive maize ubiquitin promoter. The ectopic expression of the Mx PPO transgene was investigated in the T 0 generation by Northern blot and Western blot analysis. The T 0 transgenic plants expressing the Mx PPO gene were resistant to butafenacil based on in vitro leaf disk and in vivo foliar spray tests.
- Subjects :
- Genetics
biology
Health, Toxicology and Mutagenesis
Transgene
fungi
food and beverages
General Medicine
Genetically modified crops
biology.organism_classification
Molecular biology
Genetically modified rice
Transformation (genetics)
Protoporphyrinogen oxidase
Northern blot
Myxococcus xanthus
Agronomy and Crop Science
Selectable marker
Subjects
Details
- ISSN :
- 00483575
- Volume :
- 88
- Database :
- OpenAIRE
- Journal :
- Pesticide Biochemistry and Physiology
- Accession number :
- edsair.doi...........caa33b0206b8f02fd3f52814e64d24ba